<i>Usp49</i>^-/- mice exhibit resistance to lethal HSV-1 infection.

(A) Survival (Kaplan-Meier curve) of Usp49+/+ (n = 16) and Usp49-/- (n = 16) intravenously injected with HSV-1 (2.5×106 PFU per mouse) monitored survival for 14 days. (B) ELISA analysis of IFN-β and IL-6 in the serum of Usp49+/+ and Usp49-/- mice(n = 3) intravenously injected with HSV-1 (2.5×106 PFU per mouse) for 12 hours. (C) qRT-PCR analysis of Ifnb, Il6, Ifnan and HSV-1-UL30 mRNA in the lungs (n = 3) from Usp49+/+ and Usp49-/- mice intravenously injected with HSV-1 (2.5×106 PFU per mouse) for 24 hours. (D) qRT-PCR analysis of Ifnb, Ifna4, HSV-1-UL30 mRNA and plaque assay of the brains (n = 3) from Usp49+/+ and Usp49-/- mice intranasally injected with HSV-1 (2.5×106 PFU per mouse) for 4 days. *P P P <0.001 (two-tailed t-test). Data are representative of three independent experiments (mean ± S.D. in B-D).