IFNγ/TNF responses to brief PMA/Ionomycin stimulation are greater and more multi-functional in CD3^hiCD4^- Vδ2⁺ γδT-cells from CHB compared to NC or AHB subjects.

A/B. Comparison between CHB, NC and AHB groups of effector functions in circulating γδT-cell subsets. CHB (n = 36), NC (n = 24) and AHB (n = 7) groups are compared for %IFNγ+, %IFNγ+/TNF+ and %TNF+ cells in Vδ1+ γδT-cells, Vδ2+ γδT-cells and CD3hiCD4- T-cells following 5 hours of PMA/Ionomycin stimulation. P-values between 3 groups were determined by Kruskal Wallis test (k = 3), followed by further two-way comparisons by Mann Whitney U for initial p-value below 0.05. P-values below 0.05 were considered significant and highlighted in red font for convenience. C. Correlations between early IFNγ/TNF responses in Vδ2+ γδT-cells to PMA/Ionomycin stimulation and their expression of T/NK markers. Scatter plots show %IFNγ+, %IFNγ+/TNF+ and %TNF+ cells in Vδ2+ γδT-cells (following 5 hours of PMA/Ionomycin stimulation) on the y-axis, with x-axis showing percent expression of various T/NK markers as well as Tbet/Eomes. Correlation coefficient and p-values were determined by non-parametric Spearman rank order correlation test. For convenience, red font was used to indicate significantly positive correlations with p-values D. Comparison between CHB, NC and AHB groups for multi-functionality of CD3hiCD4- T-cells following brief PMA/Ionomycin stimulation. CD3hiCD4- T-cells from CHB subjects (green bars) show greater co-expression of IFNγ, TNF and MIP1β following 5 hours of PMA/Ionomycin stimulation, compared to CD3hiCD4- T-cells from NC (white bars) and AHB (orange bars) subjects. Error bars indicate 25% and 75% interquartile ranges. P-values between 3 groups were determined by Kruskal Wallis test (k = 3), followed by further two-way comparisons by Mann Whitney U for initial p-value below 0.05. P-values below 0.05 were considered significant and highlighted in red font for convenience. Gating strategy is shown in S4 Fig.