Reduced functionality of Vγ9Vδ2 cells in LR EOC PBMCs without defects in expression level of CD3ζ and CRTAM molecules.

<p><b>A, B</b>) Vδ2⁺CD3⁺ cell fold increases in LR (n = 16) and R (n = 32) EOC PBMCs at 14 days after BrHPP (<b>A</b>) or Zol (<b>B</b>) stimulation. <b>C</b>) Percentages of IFN-γ⁺ cells in the Vδ2⁺CD3⁺ cells measured at 5 h after the stimulation of <i>ex vivo</i> LR (n = 10) and R (n = 10) PBMCs with BrHPP (3 µM) or PMA/ionomycin (PMA/iono). <b>D</b>) Percentages of IFN-γ⁺ cells (<i>left panel</i>) and TNF-α⁺ cells (<i>right panel</i>) among the Vδ2⁺CD3⁺ cells measured at 5 h after stimulations of <i>ex vivo</i> LR (n = 4) and R (n = 4) PBMCs with increasing doses of BrHPP (0.1 to 6 µM). <b>E</b>) Expression of the CD3ζ chain measured on Vγ9Vδ2 PBMCs from the LR and R groups. Percentage (<i>upper panel</i>) and MFI (<i>lower panel</i>) of CD3ζ staining in the Vδ2⁺CD3⁺ cells from LR EOC PBMCs (n = 5), R EOC PBMCs (n = 3) and R donor PBMCs (n = 3). <b>F</b>) CRTAM expression on Vγ9Vδ2 cells measured at 20 h after stimulation of LR (n = 8) and R (n = 8) EOC PBMCs with BrHPP. No stim: no stimulation.