<p>(<b>A</b>) Representative dot plots of gating strategy used and cytokine responses detected using different stimuli. Box indicated the percentage of IFN-γ+ T cells. PMA-Ionophore was used as a positive control. (<b>B</b>) Evaluation of cytokine production in both subsets (CD4 and CD8). Percentage of T cells producing IFN-γ, TNF-α or IL-2 in response to virions (ΔRT or WT+AT2) or to a pool of peptides encompassing p24 is depicted.</p
<p>Cells from MVATG18377-immunized BALB/c mice were stimulated with antigen peptide pools or an irre...
<p><b>A.</b> Distribution of cytokines produced by multifunctional or monofunctional T cells. The pe...
<p>A) <i>Left</i>, Flow cytometric analysis of intracellular IFNγ expression in naïve CD8<sup>+</sup...
<p>PBMC from all study subjects were stimulated with peptide pools, selected for positive responses ...
<p>The IFNγ cytokine profile of CD4<sup>+</sup> and CD8<sup>+</sup> T-cells was analyzed by ICS. In ...
<p>Flow cytometry data were also analyzed according to the percentage of cytokine-producing cells an...
<p>Gating scheme used to identify CD4<sup>+</sup> and CD8<sup>+</sup> T cells. Side-scatter (SSC) pa...
<p>A representative example of the analysis performed in Vac1 (left panel) and Vac2 (right panel), t...
<p>ELISpot analysis (upper row) of a negative responder (HLA-A2<sup>−</sup> recovered individual) (A...
<p>Dot plots showing individual cytokine responses in subjects from active VL (n = 8), cured VL (n =...
<p>Cells from MVATG18377-immunized C57BL/6 mice were stimulated with antigen peptide pools or an irr...
<p>A) CD4+/CD8+ T cells were gated off the side scatter (SCC) Vs CD3 gate, after gating on lymphocyt...
<p>(A) Allogeneic Th1 and Th2 cell lines from three subjects were stimulated with PMA + ionomycin fo...
<p>A single representative sample, stimulated with CMV peptide pool, depicts the gating strategy by ...
<p>A) Morphological and CD marker expression gating strategy of CD4 and CD8 T cell. B) Representativ...
<p>Cells from MVATG18377-immunized BALB/c mice were stimulated with antigen peptide pools or an irre...
<p><b>A.</b> Distribution of cytokines produced by multifunctional or monofunctional T cells. The pe...
<p>A) <i>Left</i>, Flow cytometric analysis of intracellular IFNγ expression in naïve CD8<sup>+</sup...
<p>PBMC from all study subjects were stimulated with peptide pools, selected for positive responses ...
<p>The IFNγ cytokine profile of CD4<sup>+</sup> and CD8<sup>+</sup> T-cells was analyzed by ICS. In ...
<p>Flow cytometry data were also analyzed according to the percentage of cytokine-producing cells an...
<p>Gating scheme used to identify CD4<sup>+</sup> and CD8<sup>+</sup> T cells. Side-scatter (SSC) pa...
<p>A representative example of the analysis performed in Vac1 (left panel) and Vac2 (right panel), t...
<p>ELISpot analysis (upper row) of a negative responder (HLA-A2<sup>−</sup> recovered individual) (A...
<p>Dot plots showing individual cytokine responses in subjects from active VL (n = 8), cured VL (n =...
<p>Cells from MVATG18377-immunized C57BL/6 mice were stimulated with antigen peptide pools or an irr...
<p>A) CD4+/CD8+ T cells were gated off the side scatter (SCC) Vs CD3 gate, after gating on lymphocyt...
<p>(A) Allogeneic Th1 and Th2 cell lines from three subjects were stimulated with PMA + ionomycin fo...
<p>A single representative sample, stimulated with CMV peptide pool, depicts the gating strategy by ...
<p>A) Morphological and CD marker expression gating strategy of CD4 and CD8 T cell. B) Representativ...
<p>Cells from MVATG18377-immunized BALB/c mice were stimulated with antigen peptide pools or an irre...
<p><b>A.</b> Distribution of cytokines produced by multifunctional or monofunctional T cells. The pe...
<p>A) <i>Left</i>, Flow cytometric analysis of intracellular IFNγ expression in naïve CD8<sup>+</sup...