Biodistribution of ^99mTc-TsV.

99mTc-TsV (3.7 MBq) diluted in PBS (99mTc-TsV + PBS) or neutralized with anti-hyaluronidase serum (99mTc-TsV + Anti-Hyal serum) was intramuscularly injected in Swiss mice (6–8 weeks old, 24–28 g; n = 6 per group). Radioactivity levels were measured in the heart, liver, lungs, spleen, thyroid and kidneys at 30, 60, 240 and 1440 min post-injection. The results are expressed as the percentage of injected dose/g of tissue (%ID/g). All values are presented as the mean ± S.E.M. of two independent experiments. Statistical analysis was performed using two-way ANOVA (factors: serum administration and time). Anti-hyaluronidase serum significantly affected the mean distribution of TsV to the liver (p < 0.0001), spleen (p = 0.0115), and kidneys (p = 0.0009), while time was a significant factor for TsV distribution to the heart (p < 0.0001), liver (p < 0.0001), lungs (p = 0.0095), spleen (p = 0.0008), and kidneys (p < 0.0001). A significant interaction between serum administration and time was observed in the heart (p = 0.00003), liver (p < 0.0001), spleen (p = 0.0337), and thyroid (p < 0.0001).