Hyaluronidase neutralization as a first-aid treatment for scorpion sting.

3.7 MBq of 99mTc-TsV diluted in PBS was intramuscularly injected in Swiss mice (6–8 weeks old, 24–28 g; n = 6 per group). Subsequently, anti-hyaluronidase serum was injected in the same site of 99mTc-TsV injection at different times (Anti-Hyal serum; 0, 10, and 30 min post-injection; arrows indicate the injection times). Radioactivity levels in the bloodstream were measured at 1, 5, 10, 15, 20, 30, 45, 60, 90, 120, and 240 min post-injection of 99mTc-TsV. All data are expressed as the mean percentage of the injected dose of 99mTc-TsV per gram of blood (% ID/g) ± S.E.M. of the mean. Values are representative of duplicates from two independent experiments. Statistical analysis was performed using a linear mixed model. Time (p 99mTc-TsV blood clearance.