Csf1r or Mer inhibition diminishes KC cytokine production in vitro.

(A) IL-6 (left) and TNF-α (right) as measured by quantitative PCR in freshly isolated F4/80hi KCs cultured overnight with PLX5622 and then treated with LPS for 4h. (B) IL-6 (left) and TNF-α (right) as measured by quantitative PCR in freshly isolated Mer+ KCs from wild-type mice treated in vivo with 1 week of PLX5622. (C, D) Supernatant IL-6 and TNF-α levels measured by cytometric bead array in sorted and cultured CD11bhi, Mer+Csf1r-, and Mer+Csf1r+ KCs after overnight with either (C) PLX5622 or (D) UNC2250 and then stimulated with LPS for 4h. (E) Viability of F4/80hi KCs freshly isolated and cultured for 72h with PLX5622 and M-CSF. Data represent at least 2 independent experiments with 3 to 5 mice per group. Bar graphs represent means +/- SEM and * p < 0.05.