Csf1r or Mer inhibition delays liver regeneration.

(A) Photomicrographs showing immunohistochemistry for PCNA in mouse livers (left) and quantification of PCNA+ cells counted per high power field (hpf) (right) 36h after PH. Scale bar, 100 μm. (B) Liver-to-body weight ratio (LBWR) 7d after PH in mice receiving PLX5622 (left) or AFS98 (right). (C) Serum ALT 36h after PH in mice receiving PLX5622. (D) Macroscopic photograph of livers from mice receiving PLX5622 36h after PH or sham surgery. (E) Photomicrographs of mouse livers 36h after PH stained with hematoxylin and eosin (top) or Oil red O (bottom). Scale bars, 100 μm. (F) Liver NPCs as a percentage of CD45+ cells 36h after PH or sham surgery. *p (G) Phospho-STAT3 protein expression in whole-liver lysates by Western blot after a 36h PH or sham surgery in mice treated with PLX5622 for 1 week before the surgery. (H) Serum cytokine levels 36h after PH or sham surgery as measured by cytometric bead array. (I) LBWR 7d after PH in mice receiving UNC2250. Data represent at least 2 independent experiments with 3 to 5 mice per group. Bar graphs represent means +/- SEM. *p < 0.05.