Additional file 1: of Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

Table S1. Summary of off-targeting comparison for RAG2. Target gene (RAG2) guide sequence compared to potential off-target sequence. Location on chromosome, intron or exon region and presence of PAM site is indicated. Table S2. Summary of off-targeting comparison for IL2RG. Target gene (IL2RG) guide sequence compared to potential off-target sequence. Location on chromosome, intron or exon region and presence of PAM site is indicated. Table S3. Summary of off-targeting comparison for Ig heavy chain. Target gene (Ig heavy chain) guide sequence compared to potential off-target sequence. Location on chromosome, intron or exon region and presence of PAM site is indicated. Table S4. Summary of off-targeting comparison for SCD5. Target gene (SCD5) guide sequence compared to potential off-target sequence. Location on chromosome, intron or exon region and presence of PAM site is indicated. Table S5. Summary of off-targeting results for RAG2. Five pigs were tested for each potential off-target gene. No off-targeting events were found. Table S6. Summary of off-targeting results for IL2RG. Five pigs were tested for each potential off-target gene. No off-targeting events were found. Table S7. Summary of off-targeting results for SCD5. Six pigs were tested for each potential off-target gene. No off-targeting events were found. Table S8. Embryo transfer to generate SCD5 knockout fetuses. Day 5–6 embryos were transferred into surrogates. A total of 6 fetus were obtained from one surrogate. Table S9. Genotype of SCD5 knockout fetuses. No wild-type allele was identified from the fetuses. Table S10. Summary of primers used to amplify genes tested for potential off-targeting sites as well as original RAG2 target gene. Table S11. Summary of primers used to amplify genes tested for potential off-targeting sites as well as original IL2RG target gene. Table S12. Summary of primers used to amplify genes tested for potential off-targeting sites as well as original Ig heavy chain target gene. Table S13. Summary of primers used to amplify genes tested for potential off-targeting sites as well as original SCD5 gene. (PDF 451 kb