Figure S5. Further detection of the potential off-target effects of sgRNA17â6. NC indicates the negative controls. Untransfected cells were used as negative controls. OTS indicates the experimental groups transfected with each pX458-sgRNAs. M, DNA marker. Red arrowheads indicate the expected cleaved bands by T7E1. (JPG 1060 kb
Figure S3. A, B. Examples of mutagenized loxP sites identified upon TA cloning PCR products from lox...
Figure S7. Analysis of the Rapgef5 project. PCR amplification of the genomic region of interest with...
Figure S16. Generation of a point mutation in Rims1 with ssODN donors. (a) The table details the F0 ...
Table S1. List of sgRNAs designed for targeting the intron16 and intron17 of KIT gene. Table S2. Pri...
Figure S1. Fluorescent images of porcine kidney cells 24âh after transfection of plasmid pX458-sgR...
Figure S2. Flow cytometry of porcine kidney cells 48âh after transfection of plasmid pX458-sgRNAs....
Figure S3. Images of cell clones expanded from single porcine kidney cell. One weekâs culture of s...
Oligos used to introduce sgRNA into px330. Each pair of forward and reverse primers were annealing a...
Table S1. Summary of off-targeting comparison for RAG2. Target gene (RAG2) guide sequence compared t...
Figure S6. Analysis of the Usp45 project. The figure shows the PCR amplification of the genomic regi...
Figure S1. Screening by Sanger sequencing of animals for the generation of a Syt7 conditional allele...
Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...
Figure S5. Analysis of the Syt4 project. PCR amplification of the genomic region of interest with (a...
Figure S3. showing off-target effects detection in successful inserted iPSCs. Using Cas-OFFinder, 17...
Figure S12. Examples of unexpected point mutations in the F0 animals obtained from the co-injection ...
Figure S3. A, B. Examples of mutagenized loxP sites identified upon TA cloning PCR products from lox...
Figure S7. Analysis of the Rapgef5 project. PCR amplification of the genomic region of interest with...
Figure S16. Generation of a point mutation in Rims1 with ssODN donors. (a) The table details the F0 ...
Table S1. List of sgRNAs designed for targeting the intron16 and intron17 of KIT gene. Table S2. Pri...
Figure S1. Fluorescent images of porcine kidney cells 24âh after transfection of plasmid pX458-sgR...
Figure S2. Flow cytometry of porcine kidney cells 48âh after transfection of plasmid pX458-sgRNAs....
Figure S3. Images of cell clones expanded from single porcine kidney cell. One weekâs culture of s...
Oligos used to introduce sgRNA into px330. Each pair of forward and reverse primers were annealing a...
Table S1. Summary of off-targeting comparison for RAG2. Target gene (RAG2) guide sequence compared t...
Figure S6. Analysis of the Usp45 project. The figure shows the PCR amplification of the genomic regi...
Figure S1. Screening by Sanger sequencing of animals for the generation of a Syt7 conditional allele...
Figure S1. Cytogenetic analysis of the human cell line LO2. Figure S2. NHEJ-based knock-in of ires-G...
Figure S5. Analysis of the Syt4 project. PCR amplification of the genomic region of interest with (a...
Figure S3. showing off-target effects detection in successful inserted iPSCs. Using Cas-OFFinder, 17...
Figure S12. Examples of unexpected point mutations in the F0 animals obtained from the co-injection ...
Figure S3. A, B. Examples of mutagenized loxP sites identified upon TA cloning PCR products from lox...
Figure S7. Analysis of the Rapgef5 project. PCR amplification of the genomic region of interest with...
Figure S16. Generation of a point mutation in Rims1 with ssODN donors. (a) The table details the F0 ...