S100 proteins are strongly upregulated in HS lesional skin.

qPCR for (A) S100A7 (log2FC = 3.51, **p S100A8 (log2FC = 3.41, *p S100A7A (log2FC = 6.47, **** p < 0.0001) in lesional and non-lesional HS skin. FC is expressed as average of skin samples from 3 patients. Measurements were collected in triplicate or duplicate, as allowed by RNA yield from samples. Data is shown as mean expression value compared to non-lesional skin +/- the standard error of the mean. Non-lesional and lesional samples were compared using paired t-test. (D) IF staining for S100A7 at 20X. Scale bar is 50μm. (E) IF staining for S100A8 at 20X. Scale bar is 50μm. Immunofluorescence intensity is highest in HS lesional skin, compared to non-lesional and normal skin.