Confocal images showing PolD and Pol32 localizations in a larval salivary gland (A) and an egg chamber from adult ovary (B). The genotypes are indicated to the left. In the “merged” image in B, the nucleus of the oocyte is demarcated with a rectangular box, which is shown as enlarged images to the right with chromosomes marked with an arrowhead. Note the lack of PolD signals in the mutant. Scale bars indicate 40μm.</p
<p>(A) Chromosome organization in germline nuclei in regions of wild-type (wt) and <i>hal-2</i> muta...
<p>(A-A”) Gonad from <i>ced-3(n3692)</i> adult at 72 hrs stained for F-actin (green, phalloidin) and...
<p>(<b>A-I</b>) Mouse fertilized oocyte at pronuclear stages were double-labeled with RIC8 antibody ...
A separate image is provided for the DAPI signal (in white), the anti-Pol32 signal (in red), and the...
A. DAPI stained embryos of wild-type and pol32 mutants. These are embryos collected at 0-2hr after e...
<p>(A) Stau:GFP (arrow) is localized to the posterior of wild-type stage-9 oocytes. (B) Large <i>sav...
A. Mitotic figures showing the different classes of DSBs in the mutant pol32L27/L30; polDl10/+. In t...
A. Constructs used in rescuing experiments and their effects on the mutant phenotypes. The 431aa Pol...
<p>A–C) Whole mount in situ hybridization of dissected ovaries from wild-type (A), <i>bucky ball (bu...
The normal Pol δ complex consists of PolD (black box), Pol31 (grey box) and Pol32 (white triangle). ...
<p>Immunofluorescence detection of <i>TART</i> Pol (red). DNA stained in blue except for (B). Images...
<p>(A–E) Images showing nuclear localization around nuclei of early stage germ line cells (see inset...
(A-H) Time-lapse maximum intensity projection images of live control and TS mutant oocytes upshifted...
<p>Oocytes stained with DiOC<sub>6</sub> to label ER and mitochondria (A–E). In a wild-type mid stag...
(C and C′) Two partial projections from a single optical stack though a mosaic late stage oocyte. ...
<p>(A) Chromosome organization in germline nuclei in regions of wild-type (wt) and <i>hal-2</i> muta...
<p>(A-A”) Gonad from <i>ced-3(n3692)</i> adult at 72 hrs stained for F-actin (green, phalloidin) and...
<p>(<b>A-I</b>) Mouse fertilized oocyte at pronuclear stages were double-labeled with RIC8 antibody ...
A separate image is provided for the DAPI signal (in white), the anti-Pol32 signal (in red), and the...
A. DAPI stained embryos of wild-type and pol32 mutants. These are embryos collected at 0-2hr after e...
<p>(A) Stau:GFP (arrow) is localized to the posterior of wild-type stage-9 oocytes. (B) Large <i>sav...
A. Mitotic figures showing the different classes of DSBs in the mutant pol32L27/L30; polDl10/+. In t...
A. Constructs used in rescuing experiments and their effects on the mutant phenotypes. The 431aa Pol...
<p>A–C) Whole mount in situ hybridization of dissected ovaries from wild-type (A), <i>bucky ball (bu...
The normal Pol δ complex consists of PolD (black box), Pol31 (grey box) and Pol32 (white triangle). ...
<p>Immunofluorescence detection of <i>TART</i> Pol (red). DNA stained in blue except for (B). Images...
<p>(A–E) Images showing nuclear localization around nuclei of early stage germ line cells (see inset...
(A-H) Time-lapse maximum intensity projection images of live control and TS mutant oocytes upshifted...
<p>Oocytes stained with DiOC<sub>6</sub> to label ER and mitochondria (A–E). In a wild-type mid stag...
(C and C′) Two partial projections from a single optical stack though a mosaic late stage oocyte. ...
<p>(A) Chromosome organization in germline nuclei in regions of wild-type (wt) and <i>hal-2</i> muta...
<p>(A-A”) Gonad from <i>ced-3(n3692)</i> adult at 72 hrs stained for F-actin (green, phalloidin) and...
<p>(<b>A-I</b>) Mouse fertilized oocyte at pronuclear stages were double-labeled with RIC8 antibody ...