Pol32 localization during normal development.

A separate image is provided for the DAPI signal (in white), the anti-Pol32 signal (in red), and the merged product of the two channels. A. Pol32 in ovarian tissue. A series of egg chambers (left three panels) are shown with three enlarged areas included in the right three columns of images. The nucleus of the oocyte is marked with an arrowhead. In Aa, Pol32 can be seen in the nuclei of nurse cells. In Ab, Pol32 can be seen in the nucleoplasm of the oocyte with chromosomes marked with an arrowhead. In Ac, Pol32 can be seen in nuclei of follicle cells. B. Pol32 in 0-2hr old embryos. The top left panels show images of an embryo in interphase, and the top right panels showing an embryo with condensed chromosomes. The five haploid nuclei (three polar body nuclei and the parental pronuclei) are shown underneath as enlarged images for each embryo. Ba and Bb show interphase nuclei. Bc and Bd show metaphase nuclei. C. Pol32 in syncytial cell cycles. The top row shows interphase cells with nuclear Pol32 signals. The bottom row shows mitotic cells with no Pol32 in the nucleus. Scale bars in red indicate 40μm, and 10μm in white.