Pole coalescence and polarity defects in <i>zyg-9</i> and <i>tac-1</i> mutant oocytes do not depend on prior cage structure defects.

(A-H) Time-lapse maximum intensity projection images of live control and TS mutant oocytes upshifted from 15°C to 26°C (A-D) or downshifted from 26°C to 15°C (E-H) during the multipolar stage, in oocytes expressing GFP::TBB-2 and mCherry::H2B. Oocytes depicted in F-H have cage structure defects (for 3-D images of TS mutant oocytes with cage stage defects, see S9, S10 and S11 Movies). Note that the cage stage images are maximum intensity projections of 3 focal planes (3 μm) that encompass the dis-assembling nucleus, with limited resolution due to in utero imaging. All other images are maximum intensity projections of all 20 focal plans. For images of cage stage control and mutant oocytes that project all 20 focal planes, see S5 Fig. (I-K) Number of spindle poles present at the onset of spindle shortening per oocyte (I); number of chromosome sets separated during anaphase per oocyte (J); number of oocytes with abnormal cage structures (K); number of oocytes examined indicated above each bar, number scored with each phenotype shown in parentheses inside bars with corresponding percent below. Oocytes with extra spindle poles indicated with hollow arrowheads; oocytes with chromosomes separated into three sets indicated with asterisks. Bar graphs in (I) and (J) include oocytes expressing GFP::TBB-2 (this figure) and oocytes expressing GFP::ASPM-1 (Fig 4).