Hog1 activity blocks xylose consumption.

Wildtype, catalytically inactive (hog1D144A), and truncated (hog1A844) HOG1 alleles were expressed in Y184 ira2Δhog1Δ cells and grown in batch culture for 96 hours in YPDX 6%/3%. Rate measurements were based on average and standard deviation from three biological replicates. A. Average growth rate calculated from the exponential phase of growth. B. Specific glucose consumption rate calculated from the exponential phase of growth. C. Specific xylose consumption rate calculated from the stationary phase of growth. Asterisks denote significant differences from Y184 ira2Δhog1Δ/HOG1 (paired T-Test, p < 0.05).