Role of the HOG and filamentous growth pathways in growth in galactose and effect of the inhibitory role of the HOG pathway on filamentous growth pathway outputs.

<p><b>A</b>) Serial dilutions of wild-type (PC313), <i>ste7</i>Δ (PC4928), <i>pbs2</i>Δ (PC5035) and <i>ste7</i>Δ <i>pbs2</i>Δ (PC6272) cells were spotted on YEPD and YEP-GAL media. <b>B</b>) Morphology of wild-type cells (PC538), the <i>pbs2</i>Δ mutant (PC2053), the <i>ste7</i>Δ mutant (PC4982), and the <i>ste7</i>Δ <i>pbs2</i>Δ double mutant (PC6272) grown YEP-GAL media for 24 hrs. Bar, 5 microns. <b>C</b>) Septin staining of wild-type and <i>pbs2</i>Δ cells harboring the pCdc12p-GFP plasmid. Cells were grown to mid-log phase in YEPD. <b>D</b>) Mat formation in cells lacking the filamentous growth or HOG pathways. Wild-type (PC538), <i>flo11</i>Δ (PC1029), and <i>pbs2</i>Δ (PC2053) strains were grown in YEPD medium for 16 hrs and then spotted onto low agar (0.3%) YEP-GAL medium for 3 d at 30°C. Bar, 1 cm.