A Hog1-dependent transcriptional program in G1 phase cells.

(A) GSEA analysis of differentially expressed genes in a hog1 mutant relative to a congenic wt strain. Cells were synchronized in G1 phase by centrifugal elutriation and released in fresh YPD medium for 15 min and analyzed for gene expression profiles by DNA microarrays. Up-regulated (red circles) and down-regulated (blue circles) transcripts are shown for the indicated processes. The diameter of the circle reflects the number of modulated gene transcripts in each gene set. Known functional connections between related processes are indicated (green lines). Images were generated in Cytoscape with the Enrichment Map plug-in. (B) Genome-wide promoter occupancy of Hog1 in G1 phase cells. Gene categories bound by Hog1 were determined by GO term enrichment. p-values were calculated using hypergeometric distribution. (C) Growth rate and cycloheximide (CHX; 200 μg/ml) sensitivity of wt and hog1 mutant strains. Relative growth rate was calculated as time to reach half maximal OD600 for each culture normalized to the value for the untreated WT control strain, which was 24 h of growth in SC medium at 30°C. Doubling times were calculated during the exponential phase of each strain treated or not with cycloheximide (200 μg/ml) and represented as a percentage relative to the value of the untreated WT control strain. Results are the mean of three replicates. Bars show the means +/- standard errors of the means.