MOESM3 of Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

Additional file 3. Comparison of calculated and measured values at varied initial reservoir glucose concentrations with the membrane-based fed-batch shake flask. Depiction of (a) + (b) Measured OTR; (c) + (d) Measured and calculated amount of metabolized glucose; (e) + (f) Measured and calculated amount of cell dry weight; (g) + (h) Measured and calculated amount of metabolized ammonium and accumulated glucose. For calculations the stoichiometric equation C6H12O6 + 0.57 NH3 + 2.34 O2 → 3.38 CH1.7O0.43N0.17 + 2.62 CO2 + 3.99 H2O was used. Calculations until the abrupt OTR increases are based on measured total oxygen consumed values, calculations after the abrupt OTR increase are based on the calculated glucose release; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, reservoir: 250/375/500/750 g/L glucose, blue dextran concentration: 1 g/L), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD600: 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm2; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP; Concentration values have been volume-corrected