MOESM4 of Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask

Additional file 4. Depiction of ammonium concentrations in the culture medium and reservoir of the multi-component fed-batch cultivations at varied initial glucose concentrations and 40 g/L ammonium carbonate in the reservoir. Depiction of (a) Oxygen transfer rate; (b) Ammonium concentration in the culture medium; (c) Ammonium concentration in the reservoir; Cultivation conditions: Wilms-MOPS-mineral medium (culture medium: 200 mM MOPS with 7 g/L initial ammonium sulfate, initial reservoir glucose concentrations: 250/375/500/750 g/L, 40 g/L ammonium carbonate, blue dextran concentration: 1 g/L), temperature: 37 °C, shaking frequency: 350 rpm, shaking diameter: 50 mm, initial culture medium volume: 10 mL, inoculation OD600: 0.5, reservoir filling volume: 2 mL, dialysis membrane: Reichelt 10-20 kDa, membrane area: 18.1 mm2; Strain: E. coli BL21 (DE3) pRhotHi-2-EcFbFP; Concentration values have been volume-corrected