Reduced cellular and humoral antibody response induced by r<i>Ts</i>-Hsp70 in TLR2/4^-/- mice.

<p>(A) The proliferation of splenocytes from r<i>Ts</i>-Hsp70 immunized WT, TLR2^-/-, TLR4^-/- mice upon stimulation with r<i>Ts</i>-Hsp70 was measured by MTS colorimetric assay. (B) The cytokine-secretion of splenocytes from r<i>Ts</i>-Hsp70-immunized WT, TLR2^-/- and TLR4^-/- mice upon re-stimulation of r<i>Ts</i>-Hsp70 measured by ELISA. (C) Serological anti-r<i>Ts</i>-Hsp70 IgG, IgG1 and IgG2a antibody titers against r<i>Ts</i>-Hsp70 in WT, TLR2^-/- and TLR4^-/- mice measured by ELISA. Pre-immunization control was serological anti-r<i>Ts</i>-Hsp70 IgG antibody titers of 0 days before immunization. The endpoint values are shown. Quantified data are shown as mean ± SD of three separate experiments. n = 10, * <i>p</i> < 0.05, ** <i>p</i> < 0.01, *** <i>p</i>< 0.001.