Detection of DENV strains by RT-PCR and nested PCR.
- Benjamin Lopez-Jimena (4854436)
- Michaël Bekaert (121005)
- Mohammed Bakheit (4960669)
- Sieghard Frischmann (710564)
- Pranav Patel (415128)
- Etienne Simon-Loriere (140017)
- Louis Lambrechts (241741)
- Veasna Duong (106893)
- Philippe Dussart (181796)
- Graham Harold (73273)
- Cheikh Fall (5309297)
- Oumar Faye (32488)
- Amadou Alpha Sall (169451)
- Manfred Weidmann (448254)
Publication date
May 2018
DOIAbstract
<p>(A) RT-PCR using D1 and D2 primers. (B) Serotype-specific nested PCR using D1/TS1, D1/TS3 and D1/TS4 primers to detect DENV1, DENV3 and DENV4, respectively. L: 100 bp DNA ladder (Thermo Scientific); NC: negative control (H<sub>2</sub>O); 1: KDH0030A (DENV1); 2: DJOS1.7.12 (DENV2); 3: KDH0010A (DENV3); 4: VIMFH4 (DENV4); nP: negative control nested PCR; P: negative control PCR.</p
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