<p>Lines 1–4: positive samples; 5–8: negative samples; 9–12: positive control for DENV-1, DENV-2, DENV-3 and DENV-4; NC: negative control; MW: molecular weight marker.</p
<p>Reverse transcriptase-polymerase chain reaction (RT-PCR) primers and conditions.</p
<p>Wells 1, 2 and 3 indicate negative pools for the dengue virus; well 4 reveals genomic fragment of...
<p>(A) Scatter plots of FAM and Cy5 drop intensities for a control sample (left) and picoinjected sa...
<p>PCR products were separated on agarose gel and stained with ethidium bromide to display amplified...
<p>The reverse primers DENV_R1–3 (A) and DENV_R4 (B) were specifically designed to target DENV serot...
<p>L: 500 bp ladder, 1–4: patients' samples; 5: positive control; 6: negative control.</p
<p>(A) RT-PCR using D1 and D2 primers. (B) Serotype-specific nested PCR using D1/TS1, D1/TS3 and D1/...
Immunochromatographic assay for DENV NS1Ag test for real time RT-PCR positive samples (29, 42, 50 an...
a) PCRs for AIEC. Lane M: Marker 100 bp; Lanes 1–4: AIEC positive samples; b) PCRs for L. monocytoge...
<p>PCR amplified products of <i>rol</i> B (a), <i>rol</i> C (b) and <i>nptII</i> (c) gene are shown ...
<p>Upper panel: Real Time amplification plots of a positive for endothelial NO synthase (e-NOS) and ...
<p>Positive (P) and negative controls (N) were included in each PCR assay. The expected length of th...
<p>A: The PCR product amplified from the genomic DNA using the gB primers (Lane 1) and negative cont...
<p>DENV1-3 RT-RPA assay detected DENV1-3 but not other viral RNA. DENV4 RT-RPA identified DENV2 and ...
Lane 1: PCR Negative Control (PCR Mix). Lane 2: pAE rTES-30 Clone 1. Lane 3: pAE rTES-120 Clone 1. L...
<p>Reverse transcriptase-polymerase chain reaction (RT-PCR) primers and conditions.</p
<p>Wells 1, 2 and 3 indicate negative pools for the dengue virus; well 4 reveals genomic fragment of...
<p>(A) Scatter plots of FAM and Cy5 drop intensities for a control sample (left) and picoinjected sa...
<p>PCR products were separated on agarose gel and stained with ethidium bromide to display amplified...
<p>The reverse primers DENV_R1–3 (A) and DENV_R4 (B) were specifically designed to target DENV serot...
<p>L: 500 bp ladder, 1–4: patients' samples; 5: positive control; 6: negative control.</p
<p>(A) RT-PCR using D1 and D2 primers. (B) Serotype-specific nested PCR using D1/TS1, D1/TS3 and D1/...
Immunochromatographic assay for DENV NS1Ag test for real time RT-PCR positive samples (29, 42, 50 an...
a) PCRs for AIEC. Lane M: Marker 100 bp; Lanes 1–4: AIEC positive samples; b) PCRs for L. monocytoge...
<p>PCR amplified products of <i>rol</i> B (a), <i>rol</i> C (b) and <i>nptII</i> (c) gene are shown ...
<p>Upper panel: Real Time amplification plots of a positive for endothelial NO synthase (e-NOS) and ...
<p>Positive (P) and negative controls (N) were included in each PCR assay. The expected length of th...
<p>A: The PCR product amplified from the genomic DNA using the gB primers (Lane 1) and negative cont...
<p>DENV1-3 RT-RPA assay detected DENV1-3 but not other viral RNA. DENV4 RT-RPA identified DENV2 and ...
Lane 1: PCR Negative Control (PCR Mix). Lane 2: pAE rTES-30 Clone 1. Lane 3: pAE rTES-120 Clone 1. L...
<p>Reverse transcriptase-polymerase chain reaction (RT-PCR) primers and conditions.</p
<p>Wells 1, 2 and 3 indicate negative pools for the dengue virus; well 4 reveals genomic fragment of...
<p>(A) Scatter plots of FAM and Cy5 drop intensities for a control sample (left) and picoinjected sa...