<p>Genomic locations are provided for the 5′ base of each primer based on reference virus sequences: DENV-1, US/Hawaii/1944 (GenBank: EU848545.1); DENV-2, New Guinea C Strain (GenBank: AF038403.1); DENV-3, strain H87 (GenBank: M93130.1); DENV-4, strain H241 (GenBank: AY947539.1). Predicted amplicon sizes are as follows: DENV-1, 114 bp; DENV-2, 85 bp; DENV-3, 114 bp; DENV-4, 110 bp.</p
<p>Sequences, size of the amplicon and target region of the primer and probe sets used for the qPCR ...
<p>Each strain is indicated by Genbank accession number followed by country and year of isolation. 1...
<p>(A) Maximum-likelihood trees were constructed using MEGA V5.05 software with bootstrap support of...
<p>The reverse primers DENV_R1–3 (A) and DENV_R4 (B) were specifically designed to target DENV serot...
a<p>Genomic location of strain R2B is based on the complete genomic sequence of NDV strain Mukteswar...
<p>Representative DENV strains from currently circulating genotypes considered for primer and probe ...
<p>DENV-1 nucleotide sequences from Sri Lanka were aligned with representative DENV-1 sequences from...
<p>These are compared with other recent DENV-2 isolates such as Townsville 1993 that were imported o...
This step-by-step protocol describes the cDNA synthesis, primer pools preparation and multiplex PCR ...
This step-by-step protocol describes the cDNA synthesis, primer pools preparation and multiplex PCR ...
<p>(A) PCA and phylogenetic clustering of 58 DENV4 genomes. Four subgroups were necessary to describ...
This step-by-step protocol describes the cDNA synthesis, primer pools preparation and multiplex PCR ...
This step-by-step protocol describes the cDNA synthesis, primer pools preparation and multiplex PCR ...
<p>. The schematic shows a representation of the genome for the Key West DENV-1 isolate (E = envelop...
<p>(A) RT-PCR using D1 and D2 primers. (B) Serotype-specific nested PCR using D1/TS1, D1/TS3 and D1/...
<p>Sequences, size of the amplicon and target region of the primer and probe sets used for the qPCR ...
<p>Each strain is indicated by Genbank accession number followed by country and year of isolation. 1...
<p>(A) Maximum-likelihood trees were constructed using MEGA V5.05 software with bootstrap support of...
<p>The reverse primers DENV_R1–3 (A) and DENV_R4 (B) were specifically designed to target DENV serot...
a<p>Genomic location of strain R2B is based on the complete genomic sequence of NDV strain Mukteswar...
<p>Representative DENV strains from currently circulating genotypes considered for primer and probe ...
<p>DENV-1 nucleotide sequences from Sri Lanka were aligned with representative DENV-1 sequences from...
<p>These are compared with other recent DENV-2 isolates such as Townsville 1993 that were imported o...
This step-by-step protocol describes the cDNA synthesis, primer pools preparation and multiplex PCR ...
This step-by-step protocol describes the cDNA synthesis, primer pools preparation and multiplex PCR ...
<p>(A) PCA and phylogenetic clustering of 58 DENV4 genomes. Four subgroups were necessary to describ...
This step-by-step protocol describes the cDNA synthesis, primer pools preparation and multiplex PCR ...
This step-by-step protocol describes the cDNA synthesis, primer pools preparation and multiplex PCR ...
<p>. The schematic shows a representation of the genome for the Key West DENV-1 isolate (E = envelop...
<p>(A) RT-PCR using D1 and D2 primers. (B) Serotype-specific nested PCR using D1/TS1, D1/TS3 and D1/...
<p>Sequences, size of the amplicon and target region of the primer and probe sets used for the qPCR ...
<p>Each strain is indicated by Genbank accession number followed by country and year of isolation. 1...
<p>(A) Maximum-likelihood trees were constructed using MEGA V5.05 software with bootstrap support of...