Knockdown of genes in calcium pathway in the PG affects the periodicity of eclosion.

<p>(A) Knockdown of calcium channels <i>cacophony</i> (a), <i>Ca-alpha1D</i> (b), and of IP3 receptor (<i>IP3R</i>) (c) in the PG lengthen the periodicity of eclosion with respect to controls (d). Records show time course of emergence of a single population in DD (left) and corresponding MESA analyses (right); principal periodicity is indicated. (B) Knockdown of <i>SERCA</i> in the PG shortens the periodicity of eclosion (a) compared to control (b). (C) Knockdown of <i>CaMKII</i> (a) and <i>CASK</i> (b) in the PG lengthens the periodicity of eclosion. (D) Free-running periodicity for results shown in A-C. Each point indicates results from separate experiments; average is indicated by horizontal line; different letters indicate statistically different groups (<i>p</i><0.05; one-way ANOVA, Tukey’s <i>post hoc</i> multiple comparison analyses). (E) Average rhythmicity index (RI) values (± SEM) for results shown in A-C. Numbers in parenthesis indicate number of separate experiments; different letters indicate statistically different groups (<i>p</i><0.05; one-way ANOVA, Tukey’s <i>post hoc</i> multiple comparison analyses). In A-C, GAL4 driver was used in combination with UAS-<i>dcr2</i> to potentiate effectiveness of RNAi knockdown. Flies bearing only UAS-RNAi transgenes express normal rhythmicity of emergence (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007433#pgen.1007433.s001" target="_blank">S1 Fig</a>).