Additional file 5: of Altering cortical input unmasks synaptic phenotypes in the YAC128 cortico-striatal co-culture model of Huntington disease

Figure S4. Increased thin spine density and reduced mushroom spine head diameter in DIV21 YAC128 cortical neurons. WT and YAC128 pure cortical cultures were fixed at DIV21 and subjected to in vitro DiI DiOlistic dye labeling for spine analysis. (A) Sample images of DiI-stained spines on cortical dendrites (scale bar = 5 μm). No significant differences in (Bi) total, (Bii) mushroom, or (Biii) stubby spine densities were observed in YAC128 cortical neurons. (Biv) Increased thin spine density and (Bv) reduced mushroom spine head diameter were measured in YAC128 cortical neurons compared to WT [n = 30(3); Student’s t test; *p < 0.05]. (TIF 288 kb