Additional file 3: of Loss of CREST leads to neuroinflammatory responses and ALS-like motor defects in mice

Figure S2. Loss of CREST Function Does not Affect the Formation of Stress Granules and the Axon Length in Cultured Neurons in vitro, related to Fig. 2. (A) Representative Immunofluorescence staining of stress granule marker YB1 (red) in the treatment of nothing as control (left) or 50μM sodium arsenite (SA) for 1h (right). Arrows indicate the cells without stress granules (SGs) in the treatment of SA. Arrowheads indicate the cells with SGs. Scale bar, 15μm. (B) Quantification of the fraction of cells with SGs in cultured neurons isolated from embryotic Crest −/−, Crest +/− mice and WT littermates. (C and D) Representative Immunofluorescence images (C) and the total length quantification (D) of SMI312-positive (red) axons in cultured neurons expressing GFP (C, top), shCREST labeled by GFP (C, middle) and WT human CREST labeled by GFP (green) (C, bottom). Scale bar, 100μm. Error bars represent SEM. One-way ANOVA. (TIF 13203 kb