Additional file 4: of Altering cortical input unmasks synaptic phenotypes in the YAC128 cortico-striatal co-culture model of Huntington disease

Figure S3. Reduced dendritic length and complexity in co-cultured YAC128 MSNs are developmental phenotypes. WT and YAC128 co-cultures were generated at either a 1:1 or 1:3 CS ratio and processed at DIV14, 18, and 21 for DARPP32 immunocytochemistry, imaging, and dendritic analysis. (A) Sample images of MSN dendritic traces generated in NeuronStudio (scale bar = 15 μm). A developmental increase in (Bi, Bii, Biii) dendritic complexity by Sholl analysis and (Biv) total dendritic length are impaired after DIV14 in co-cultured YAC128 MSNs compared to WT. Post-hoc statistical significance for Sholl analysis is shown only for WT 1:1 vs. YAC128 1:1 (*) or WT 1:3 vs. YAC128 1:3 (#) comparisons [n = 32(4); two-way ANOVA with Bonferroni post-hoc analysis; *p < 0.05, **p < 0.01, ***p < 0.001]. (TIF 875 kb