In vivo metastasis assay.

<p><i>In vivo</i> assays for metastasis in rat lung with parental and transformed cell lines. Cells (1x10⁶) were injected into tail vein of immunodeficient rats. After 45 days the animals were euthanized and the lungs were analyzed. (A) Lungs and H&E lung sections of animals injected with the MCF10A, MCF10A-empty vector and MCF10A/CD90+ cell lines. (B) Lungs and H&E lung sections of animals injected with the Hs578T, Hs578T non-target and Hs578T/shCD90 cell lines. C) Number of lesions on the surface of the lungs of the animals injected with the cells of the strains MCF10A, MCF10A-empty vector and MCF10A/CD90+. (D) Number of lesions on the surface of the lungs of the animals injected with the cells of the strains Hs578T, Hs578T non-target and Hs578T/shCD90. ANOVA test followed by Tukey’s test for post hoc comparison were used for statistical analysis. Asterisks show statistically significant expression differences (p <0.05). The experiments were performed in two independent experiments in triplicate.