Determining the functional GATA3 sites in the CRTh2 proximal promoter.

<p>Recombinant GATA3 and NFAT1 were used to characterize binding. Mutated CRTh2 proximal oligonucleotides were used as competitors <b>(A)</b> and labelled as probes <b>(B)</b>. Competitors and super-shifting antibodies used are noted above the corresponding lanes. Antibodies denoted as α. G1, G2, and G3 refer to the GATA3 site that has been mutated in each case.