Identification of a CRE/AP-1-like motif and GATA-binding site within the Syncytin-2 promoter.

<p><b>(A)</b> Extended probe 5 (termed WT) (from-211 to-177) (indicated in the upper part of the panel) was used for EMSA analyses and highlights the CRE/AP-1-like motif and the potential GATA-binding site in the Syncytin-2 promoter. Nuclear extracts from BeWo cells treated (T) or not (NT) with forskolin for 15 min were pre-incubated with different concentrations of cold specific or non-specific oligonucleotides prior to addition of the labeled WT probe. (<b>B</b>) EMSA analyses of nuclear extracts from BeWo cells stimulated with forskolin at different time points and incubated with the WT probe. <b>(C)</b> Representation of the various oligonucleotides bearing mutated sequences (nucleotides in italic) used in EMSA analyses. <b>(D-E)</b> Nuclear extracts from non-treated (NT) or forskolin-treated (T) BeWo cells were incubated with the WT probe and excess (100X) cold WT or mutated oligonucleotides presented in (<b>C</b>). Specific DNA-protein complexes are indicated on left side of panels. (<b>F</b>) Nuclear extracts from primary villous cytotrophoblasts cultured for 24 and 96 hours were incubated with the WT probe and with or without excess (100X) cold WT oligonucleotide.