Effect of lobeglitazone (Lobe) on insulin resistance in high-fat diet (HFD)-fed mice.

<p>(A) Serum glucose, (B) serum insulin, (C) homeostatic model assessment of insulin resistance (HOMA-IR), (D) glucose tolerance test, and (E) insulin tolerance test in normal diet (ND), non-treated HFD, Lobe-treated (1 or 5 mg/kg/d) HFD, and Lobe-only mice. (F) Representative microphotographs of immunostained insulin in pancreatic sections from each group. Scale bar = 100 μm. Western blot for (G) glucose transporter 4 (GLUT4) and (H) insulin receptor (IR) expression in the liver for each group. Quantification of GLUT4 and IR expression from western blot analysis. Densitometry values are normalized to β-actin and represented as arbitrary units. (I) Serum lipocalin-2 concentrations obtained by enzyme-linked immunosorbent assay. Data are presented as mean ± SEM. *<i>P</i> <0.05 vs. ND-fed mice; †<i>P</i> <0.05 vs. HFD-fed mice. AUC, area under the curve.