TGFβ1 increases the gene expression of NSCLC stem-like cell markers and colony formation ability in TGFβ1 sensitive NSCLC lines.

<p><b>(A)</b> Real-time PCR showed that 2.5 ng/ml TGFβ1 treatment for two weeks significantly increased Oct4 and Sox2 mRNA expression in A549, NCI-H358 and NCI-H1993 cells compared to untreated cells (** <i>P</i><0.01, *** <i>P</i><0.001). <b>(B)</b> Oct4, Nanog and Sox2 expression remained same before and after TGFβ1 treatment. <b>(C)</b> 2.5 ng/ml TGFβ1 treatment for two weeks significantly enhanced anchorage-dependent colony formation ability of A549, NCI-H358 and NCI-H1993 cells but not for NCI-H1975, NCI-H1650 and HCC827 cells (* <i>P</i><0.05, ** <i>P</i><0.01).