NTM cells treated with TGFβ1 show increased expression of COL1A1 and DNMT1 and decreased expression of RASAL1.

<p>We examined the effect of 10ng/ml TGFβ1 on NTM cells after 24 hour treatment. <b>A.</b> There was no significant change in cell viability of cells treated with 10ng/ml TGFβ1. <b>B.</b> Upon investigation of mRNA expression of COL1A1, DNMT1 and RASAL1 by qPCR, we found that COL1A1 (P<0.05) and DNMT1 were increased and RASAL1 (P<0.05) was decreased in the cells treated with TGFβ1. <b>C.</b> We examined RASAL1 protein by Western blotting and conducted densitometry on the blots. We found that, like the mRNA expression, TGFβ1 decreased RASAL1 expression in NTM cells. *P<0.05, **P<0.01 Results are from 3 independent experiments.