Additional file 5: of Transposable element insertions shape gene regulation and melanin production in a fungal pathogen of wheat

Zmr1 regulates melanin biosynthesis in Z. tritici. (A) Three independent Zmr1 disruptant mutants in the 3D1 (#46, #48, and #2.1) and in the 3D7 (#3, #6, and #100) backgrounds lack melanin. Pictures of 10-day-old wild-type 3D7 and 3D7Δzmr1 colonies. (B) Melanized and albino pycnidia of 3D7 and 3D7Δzmr1, respectively, on wheat leaves of the cultivar Drifter, 21 dpi. (C) Expression values of the genes in the DHN melanin biosynthesis pathway (Pks1 = polyketide synthase 1; Thr1 = 1,3,8-trihydroxynaphthalene reductase; Zmr1 = Zymoseptoria melanin regulation 1; Ayg1 = Homolog of Aspergillus yellowish green) for the wild-type and Δzmr1 in the 3D1 and 3D7 backgrounds, respectively. Mean of TMM (trimmed mean of M values) normalized log2 CPM (counts per million mapped reads) values of three independent replicates with their standard deviation are plotted. Asterisks indicate statistical differences between the wild-type and the mutant (p value ≤ 0.05, FDR ≤ 0.05). (TIF 1614 kb