Additional file 14: of Transposable element insertions shape gene regulation and melanin production in a fungal pathogen of wheat

No insertions of transposable elements were identified upstream of Zmr1 in IPO323. A) Melanization levels of the reference strain IPO323 from 8 to 12 days post inoculation. Bars represent means and standard errors of the mean gray value based on at least 60 colonies. Representative pictures of both strains are shown below the bar plot for all the time points. The experiment was performed two times with similar results. Gray value scale (0 = black, 255 = white) is shown on the left. B) Synteny plot of the QTL between 3D7, 3D1 and the reference strain IPO323 showing the insertion of an island of transposable elements 1.8 kb upstream of the start codon of Zmr1 only in 3D1. Brown lines indicate collinear sequences. The positions of genes and transposable elements are shown using purple arrows and blue bars, respectively. Vertical yellow lines indicate simple repeats. The different shades of blue represent different classes of transposable elements that were classified according to the three-letter code described in Wicker et al., [100]. The first letter indicates the class (R = RNA class and D = DNA class), the second letter indicates the order (L = LTR, I = Line, T= TIR) and the third letter indicates the superfamily (G = Gypsy, I = I, X = unknown). (TIF 100521 kb