Localization of the viral N protein and dsRNA in Marc-145 cells infected with PRRSV.

<p>At the 24 h time point, Marc-145 cells were fixed and performed for immunofluorescence as mentioned in the Materials and Methods after PRRSV infection at an MOI of 1. Images of samples were visualized using a Nikon TE2000-E inverted confocal microscope at 63× magnification. (A)—(H) The cellular nuclei were dyed with DAPI (blue). Cells were co-stained with the J2 monoclonal anti-dsRNA antibody and (A) the anti-calnexin monoclonal antibody, (B) the anti-LC3 monoclonal antibody, (C) LysoTracker Red or (D) MitoTracker Red. Cells were costained with the monoclonal anti-NS1 antibody and (E) the anti-calnexin monoclonal antibody, (F) the anti-LC3 monoclonal antibody, (G) LysoTracker Red or (H) MitoTracker Red. Scale bars, 10 μm. (I) The graph showing Pearson’s correlation coefficients for each markers in (A)~(H), values above 0.5 were considered as colocalization.