<p>Detection of the N viral protein in PRRSV infected SJPL cells by immunofluorescence. SJPL cells were infected with 0.5 MOI of PRRSV for 4 hours then incubated with DMEM culture medium alone (DMEM) (A) or either a DMEM culture medium fraction of <1 kDa (DMEM <1 kDa) (B) or a <i>AppΔapxIΔapxIIC</i> cell culture supernatant <1 kDa fraction (<i>App</i><1 kDa) (C) added to complete SJPL culture medium for 48 hours. White scale bar represents 200 µm. Pictures were taken at 100X magnification.</p
(A) PK-15 cells were mock-infected or infected with PRV-GFP (MOI = 0.1) for 24 hours. PI(3,5)P2 (red...
<p>(A). Gradient purifier RSV (∼10<sup>5</sup> particles/50 µl) was resolved on the SDS-PAGE, follow...
<p>(A, B and C) Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with...
<p>Cells grown in 6-well plates were infected with PRRSV GD-XH (0.05 MOI) for 2 h at 37°C and then c...
<p>At the 24 h time point, Marc-145 cells were fixed and performed for immunofluorescence as mention...
<p>SJPL (A and B) and MARC-145 cells (C and D) were infected with or without PRRSV at an MOI of 0.5 ...
<p>qRT-PCR results expressed in relative expression (ΔΔCT) for IFNα (A), IFNβ (B) and IFNγ (C) in SJ...
<p>All experiences were performed at least 2 times.</p><p>Statistical <i>P</i> value compared to <i>...
<p>(A) Schematic of the experimental design for the detection of PRRSV infection in different transg...
<p>(A) Cells infected with parental viruses (PRV Becker, 180) or gM-pHluorin expressing viruses (PRV...
<p>Marc-145 cells were transfected with 0.5, 1, 1.5 μg of pVAX-mVIP or pVAX-1 plasmids, individually...
<p>Marc-poFcγRII cells were exposed to PRRSV-antibody complex, prepared by mixing each dilution of t...
<p>PRRSV at an MOI of 1 was transfected into Marc-145 cells, and at 24hours post-infection, cells we...
<p>The infection rates following treatment with PRRSV-specific antibody was determined by immunofluo...
<p><b>a</b>. Plaque size measurements. BSC-40 cells were infected with the indicated viruses and cul...
(A) PK-15 cells were mock-infected or infected with PRV-GFP (MOI = 0.1) for 24 hours. PI(3,5)P2 (red...
<p>(A). Gradient purifier RSV (∼10<sup>5</sup> particles/50 µl) was resolved on the SDS-PAGE, follow...
<p>(A, B and C) Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with...
<p>Cells grown in 6-well plates were infected with PRRSV GD-XH (0.05 MOI) for 2 h at 37°C and then c...
<p>At the 24 h time point, Marc-145 cells were fixed and performed for immunofluorescence as mention...
<p>SJPL (A and B) and MARC-145 cells (C and D) were infected with or without PRRSV at an MOI of 0.5 ...
<p>qRT-PCR results expressed in relative expression (ΔΔCT) for IFNα (A), IFNβ (B) and IFNγ (C) in SJ...
<p>All experiences were performed at least 2 times.</p><p>Statistical <i>P</i> value compared to <i>...
<p>(A) Schematic of the experimental design for the detection of PRRSV infection in different transg...
<p>(A) Cells infected with parental viruses (PRV Becker, 180) or gM-pHluorin expressing viruses (PRV...
<p>Marc-145 cells were transfected with 0.5, 1, 1.5 μg of pVAX-mVIP or pVAX-1 plasmids, individually...
<p>Marc-poFcγRII cells were exposed to PRRSV-antibody complex, prepared by mixing each dilution of t...
<p>PRRSV at an MOI of 1 was transfected into Marc-145 cells, and at 24hours post-infection, cells we...
<p>The infection rates following treatment with PRRSV-specific antibody was determined by immunofluo...
<p><b>a</b>. Plaque size measurements. BSC-40 cells were infected with the indicated viruses and cul...
(A) PK-15 cells were mock-infected or infected with PRV-GFP (MOI = 0.1) for 24 hours. PI(3,5)P2 (red...
<p>(A). Gradient purifier RSV (∼10<sup>5</sup> particles/50 µl) was resolved on the SDS-PAGE, follow...
<p>(A, B and C) Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with...