MTX treatment has not effect on the expression of IL-6 cytokine in HSF exposed to PIC.

<p>HSF were stimulated by PIC 100μg/mL and treated or not with MTX 1μM. <b>A)</b> IL-6 mRNA levels from HSF stimulated by PIC100μg/mL for 6 hours and 24 hours in the absence and presence of MTX 1μM treatment were evaluated by qRT-PCR. <b>B)</b> Supernatants were harvested after 6 hours and 24 hours and levels of IL-6 were measured by ELISA assay. All experiments were done in quadruplicates and results are expressed as mean ± standard error. *: p-values ≤ 0.05, **: p-values ≤ 0.01, ***: p-values ≤ 0.001 and ****: p-values ≤ 0.0001.