MTX treatment does not modulate the expression of RANKL in HSF exposed to PIC.

<p><b>A)</b> mRNA levels of RANKL from HSF stimulated by PIC100μg/mL in the absence and presence of MTX 1μM treatment were measured by qRT-PCR. <b>B)</b> Culture supernatants were harvested after 24 hours, 48 hours and 72 hours and RANKL protein levels were measured by ELISA assay. All experiments were done in triplicates and results are expressed as mean ± standard error. *: p-values ≤ 0.05, **: p-values ≤ 0.01, ***: p-values ≤ 0.001 and ****: p-values ≤ 0.0001.