Effects of MTX with or without (FO) and/or genistein (Gen) supplementation on expression of osteoclastogenesis-regulatory or related genes.

<p>Levels of mRNA expression in metaphysis bones of treated rats as quantitated by real time RT-PCR: (<b>A</b>) RANKL/OPG ratio, (<b>B</b>) TNF-α, and (<b>C</b>) IL-6. (<b>D</b>) Levels (pg/mL) of circulating IL-6 protein in plasma samples of treated rats as measured by multiplex cytokine assay. Labelled means without a common letter differ (P<0.05).