Additional file 2: of Mutations in dock1 disrupt early Schwann cell development

Figure S2. A) The stl365 allele was generated by a TALEN that resulted in one base pair insertion causing an early stop. B) Genotyping assay for the stl365 lesion. The PCR amplified product is digested with EcoRV and run on a 3% agarose gel. C) The stl366 allele was generated by a TALEN that resulted in a 13 base-pair deletion causing an early stop. D) Genotyping assay for the stl366 lesion. The PCR amplified product is digested with HpyCH4III and run on a 3% agarose gel. E) RT-PCR for dock1 on adult PLLn cDNA shows dock1 is expressed in the PLLn. F) Control reaction performed with Milli-q water as a substrate. (PDF 362 kb