Comparison of phenotypes of gain-of-function and loss-of-function of <i>wg</i>, a negative regulator of eye development, using <i>dpp</i>-Gal4 with newly identified <i>dpp-</i>enhancer Gal4 lines.

<p>(A-B) wild-type (A) third instar larval eye imaginal disc stained for Dlg (Blue), Elav (Red). (C-H) Gain-of-function of <i>wg</i> using GFP tagged <i>wg</i> (UAS -<i>wg</i> -GFP) with (C, D) <i>dpp</i>-Gal4, (E, F) <i>GMR18D08-</i>Gal4, (G, H) <i>GMR17E04</i>-Gal4 driver. Note that <i>dpp</i>-Gal4 driven GFP (<i>dpp>wg</i>-GFP) result in a “no-eye” phenotype as seen in (C) the imaginal disc and (D) the adult eye. The <i>GMR18D08</i>><i>wg</i>-GFP results in the (E) near wild-type eye imaginal disc and (F) reduced adult eye with preferential loss of ventral eye. The <i>GMR17E04</i>><i>wg</i>-GFP results (G) in slightly reduced eye disc and (H) significantly reduced adult eye due to preferential loss of ventral eye. (I-N) Loss-of-function of <i>wg</i> using UAS-<i>wg</i>^<i>RNAi</i> transgene driven by (I, J) <i>dpp</i>-Gal4, (K, L) <i>GMR18D08</i>-Gal4, (M, N) <i>GMR17E04</i>-Gal4 drivers. Note that (I, J) <i>dpp</i>><i>wg</i> ^<i>RNAi</i> results in slight enlarged eye disc and the adult eye, (K, L) <i>GMR18D08</i>><i>wg</i> ^<i>RNAi</i> results in eye field enlargement as seen in (I) the eye disc and (J) the adult eye, (M, N) <i>GMR17E04</i>><i>wg</i> ^<i>RNAi</i> results in subtle enlargement of (M) eye-antennal imaginal disc and the (N) adult eye.