Effects of NA9 on Wingless (Wg), <i>hedgehog</i> (<i>hh</i>) and <i>decapentaplegic</i> (<i>dpp</i>) expression during eye development.

Third instar larval eye discs immunostained with (A, B) anti-Wingless (Wg) and anti-Elav (marks photoreceptor neurons). (C, D) Anti-βGal stainings (red) report on dpp expression. (E, F) Anti-βGal stainings (red) report on hh expression. DAPI stainings mark the nuclei. GFP delineates the areas of ey-Gal4 activity. The following genotypes were analyzed: (A) ey-Gal4/+; UAS-GFP.nls/+ referred to as CTL. (B) ey-Gal4/UAS-NA9; UAS-GFP.nls/+ referred to as NA9. (C) ey-Gal4/dpp-LacZ referred to as CTL. (D) ey-Gal4, UAS-NA9/dpp-LacZ referred to as NA9. (E) ey-Gal4/+; hh-LacZ/+ referred to as CTL. (F) ey-Gal4, UAS-NA9/+; hh-LacZ/+ referred to as NA9. (TIF)