gp70-V1V2 protein and cyclic V2- and V3-peptide-specific Ab responses in guinea pigs.

<p><b>(A-C)</b> Ab responses to gp70-V1V2 AE.92TH023 and gp70-V1V2 B.Case A2 proteins were evaluated by ELISA. Ab responses for the cyclic V2 (<b>D-F</b>) and V3 peptides (<b>G-I</b>) were measured by SPR. In each panel, comparisons of epitope-specific Ab responses elicited in animals immunized with vaccines formulated with MF59 or AH are shown: <b>(A, D, G)</b> monovalent 6240.B (Grp 1 vs. Grp4); <b>(B, E, H)</b> A244.AE (Grp2 vs. Grp5) and <b>(C, F, I)</b> bivalent (Grp3 vs. Grp6). <b>(A-C)</b> Ab responses to gp70-V1V2 by ELISA; (<b>D-F</b>) Ab responses to cyclic V2 peptides by SPR; (<b>G-I</b>) Ab responses to cyclic V3 peptides by SPR. Each symbol for the ELISA represents the mean end-point titer for an individual animal analyzed in triplicate. The SPR assays were conducted with 4 replicates and are represented as the response units. The values shown in each panel represent the geometric mean response units for each group. Differences between groups (MF59 vs. AH) were compared using Wilcoxon rank-sum test with confidence level of p < 0.05. The p values are provided above groups that showed statistically significant differences.