Volcano plots showing miRNA expression changes as obtained by RNAseq.

<p>A) day 1 after challenge, B) day 3 after challenge, C) day 14 after challenge. x-axes show the log₂FC values of post-challenge time points vs. unchallenged controls. 50% up- or down-regulation is denoted by vertical dotted lines (log₂FC > 0.585, log₂FC < -0.585). y-axes show the -log₁₀ transformed <i>p</i>-values obtained from <i>F</i>-tests. <i>p</i> = 0.05 is denoted by a horizontal red line. A horizontal blue line denotes the <i>p</i>-value limit for significant differential expression when controlling for FDR; as shown, only on day 3 after challenge are there any miRNAs that are significantly differentially expressed after this correction. miRNAs that pass the criteria for differential expression are marked with a number denoting their identity; miRNAs that appear in more than one volcano plot are marked with the same number in all plots. 1 ssc-miR-205; 2 ssc-miR-34c; 3 ssc-miR-671-5p; 4 ssc-miR-146a-5p; 5 ssc-miR-2366; 6 ssc-miR-365-5p; 7 ssc-miR-146b; 8 ssc-miR-708-5p; 9 ssc-miR-92b-5p; 10 ssc-miR-708-3p; 11 mmu-miR-34b-3p; 12 mmu-miR-34b-5p; 13 ssc-miR-339-3p; 14 ssc-miR-196b-5p; 15 ssc-miR-193a-3p; 16 ssc-miR-4334-3p; 17 ssc-miR-133b; 18 ssc-miR-217; 19 ssc-miR-331-3p; 20 ssc-miR-216; 21 ssc-miR-187; 22 ssc-miR-144; 23 ssc-miR-296-3p; 24 ssc-miR-92b-3p; 25 ssc-miR-1343; 26 hsa-miR-375; 27 ssc-miR-744; 28 ssc-miR-7134-5p; 29 ssc-miR-190b; 30 ssc-miR-2320-3p; 31 ssc-miR-671-3p; 32 ssc-miR-128; 33 ssc-miR-328; 34 ssc-miR-30c-1-3p; 35 ssc-miR-1296-5p; 36 ssc-miR-149; 37 ssc-miR-183; 38 ssc-miR-129b; 39 ssc-miR-221-5p; 40 ssc-miR-29b; 41 ssc-miR-142-5p; 42 ssc-miR-504; 43 ssc-miR-20a; 44 ssc-miR-1249; 45 ssc-miR-7139-3p; 46 ssc-miR-486; 47 ssc-miR-451; 48 ssc-miR-335; 49 ssc-miR-215.