MiRNAs profiling between hypoxic and normoxic HPASMC.

<p>A, B: The accuracy for quantification of miR-199a-3p (A) and miR-210 (B) was evaluated in a high-throughput RT reaction. The number of pooled RT primers in a single RT reaction ranged from 1∼360. C: Scatter plot of relative expression level of 721 miRNAs determined by S-Poly(T) qPCR. The relative expression of miRNA was calculated as 2^∧-ΔCt and presented in the logarithmic scale. The data on the line across both X and Y axes represented the constitutively expressed miRNAs. D, E: Significantly up-regulated and down-regulated miRNAs. The expression of miRNAs was shown as fold change between hypoxia and normoxia. F: Scatter plot of the relative expression level of 246 miRNAs detected by LNA-based miRNA microarray. The relative expression of miRNA was calculated as Hy3/Hy5 ratio and the data on the line across both X and Y axes represented the constitutively expressed miRNAs. G: Heat map showing the expression of miRNAs that are differentially expressed in hypoxic HPASMC compared with normoxic HPASMC. Red color denotes induction and blue color denotes suppression. H: Significantly up-regulated miRNAs verified by real-time PCR. The expression of miRNAs was shown as fold change between hypoxia and normoxia. Each bar represents mean ± SD. Statistical significance was determined using two-tailed Student’s t-test. * P<0.05.