Elderly LPS/IFN-γ-MoDCs induce greater T cell proliferation at lower DC: T cell ratios.

<p>Young and elderly immature/unstimulated and LPS/IFN-γ-stimulated MoDCs were co-cultured with allogeneic, CFSE-labelled young T cells at DC: T cell ratios of 1:5, 1:20 and 1:50 for 5–8 days, and T cell proliferation analysed via flow cytometry as per <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0195313#pone.0195313.s002" target="_blank">S2A–S2E Fig</a>. Percentages of young CD4⁺ (A) and CD8⁺ T cell (B) proliferation induced by LPS/IFN-γ-MoDCs were compared to those induced by age-matched unstimulated MoDCs. Statistical comparisons were also performed between young and elderly volunteers within each condition. Data shown as individual values, n = 4–16 young volunteers’ MoDCs, n = 9–22 elderly volunteers’ MoDCs, * = p<0.05 comparing LPS/IFN-γ-MoDCs to age-matched unstimulated MoDCs.