Aged splenic DCs and APCs exhibit a similar ability to prime allogeneic T cell responses both in vitro and in vivo.

<p>A: Splenic APCs and CD11c⁺ DCs, purified from aged or young CBA mice, were cultured with C57BL/6 T cells and cellular proliferation and IFN-γ measured. Similar results were noted in two independent experiments and with an alternate donor (C57BL/6) and recipient T cell (BALB/C) combination. B: Aged or young splenic CBA DCs were injected into C57BL/6 mice. 10 days later spleens were harvested from the groups and cultured with irradiated CBA spleen cells, and cellular proliferation and IFN-γ measured. The change from T cells from mice that were not immunized is shown. There were no significant differences noted between mice injected with either young or aged allogeneic DCs. Representative data from one experiment, which was repeated with consistent results.