Additional file 2: of Structural and mechanistic aspects influencing the ADAM10-mediated shedding of the prion protein

(.jpg) Species specificity of the sPrPG228 antibody. (A) Comparison of the C-terminal amino acid sequence of PrPC in mouse (Mus musculus), rat (Rattus norvegicus) and rabbit (Oryctolagus cuniculus) (source: www.uniprot.org ). “NH 2 -…-” indicates the N-terminal direction, “-GPI” the C-terminal GPI-anchor attachment site. Asterisks indicate position of ADAM10-mediated shedding in mice and rats with Gly228 representing the new C-terminus of shed PrP. Note the sequence difference compared with rabbit PrPC. (B) Western blot analysis of forebrain homogenates from different murine models (tga20, Prnp0/0, wild-type (C57BL/6)) as well as from rat and rabbit. As expected for its epitope, the sPrPG228 antibody detects sPrP in mouse (tga20 and wild-type) and rat, whereas the brain sample of rabbit only presents an immunoglobulin light chain (rb Ig-LC) signal at 25 kDa resulting from the anti-rabbit secondary antibody used for detection. Re-probing the blot with POM2 antibody reveals expression levels of PrPC. (JPEG 388 kb