Additional file 2: Figure S2. of Two alternative pathways for generating transmissible prion disease de novo

PK-resistance and conformational stability of atypical PrPres and PrPSc. a Analysis of PK-resistance. Brain material from S05-inoculated animals that contained predominantly PrPSc (upper panel) or atypical PrPres (lower panel) were treated with increasing concentration of glycerol-free proteinase and analyzed by Western blot. b Analysis of conformational stability. Brain materials from S05-inoculated animals that contained predominantly PrPSc (upper panel) or atypical PrPres (lower panel) were incubated with increasing concentrations of GdnHCl, digested with PK and analyzed by Western blot. Animals from the second passage of S05 were used. Western blots were stained with 3F4 or SAF-84 antibody as indicated. (TIF 1302Â kb